rabbit polyclonal anti aldh1a2  (Proteintech)

Journal: iScience

Article Title: Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression

doi: 10.1016/j.isci.2023.108142

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Article Snippet: Rabbit polyclonal anti-ALDH1A2 , Proteintech , Cat #: 13951; RRID:AB_2224033.

Techniques: Virus, Recombinant, Activity Assay, SYBR Green Assay, Software

Journal: Cell reports

Article Title: Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis

doi: 10.1016/j.celrep.2021.109885

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-ALDH1A2 , Sigma-Aldrich , Cat#HPA010022; RRID: AB_1844723.

Techniques: Recombinant, Microarray, Real-time Polymerase Chain Reaction, Software

Journal: Scientific Reports

Article Title: Global miRNA/proteomic analyses identify miRNAs at 14q32 and 3p21, which contribute to features of chronic iron-exposed fallopian tube epithelial cells

doi: 10.1038/s41598-021-85342-y

Figure Lengend Snippet: miR-138-5p overexpression partially regulates stem cell marker hTERT transcript levels, but does not alter another stem cell marker ALDH1A2. ( a ) Real-time PCR analysis of miR-138-5p after isolating total miRNAs from miR-138-5p transfected 250 nM FAC-treated cells relative to control transfected FAC-treated and Untreated FT194 cells, at days 122, 125 and 129 of FAC treatment (p = 35, 36, and 37) to validate the overexpression. ( b ) Western blotting was performed for cell lysates collected from miR-138-5p transfected and control transfected Untreated and FAC-treated FT194 cells to analyze ALDH1A2 levels at days 119, 122, and 126 of FAC treatment ( p = 35–37). White space between cropped blots delineate different antibody applications to the same blot. The full-length uncropped blots are displayed in the Supplementary Information File. ( c ) Western blotting was performed using cell lysates collected from control or miR-432-5p or miR-127-3p, transfected Untreated and FAC-treated FT194 cells with the indicated antibodies at days 123, 131, and 137 of FAC treatment ( p = 35, 37, and 38). White space between cropped blots delineate different antibody applications to the same blot. The full-length uncropped blots are displayed in the Supplementary Information File. ( d ) The predicted miR-138-5p binding site in the TERT sequence is shown. ( e ) Real-time PCR analysis of TERT in miR-138-5p transfected FAC-treated FT194 cells, relative to control transfected Untreated and FAC-treated FT194 cells after isolating miRNAs at days 119, 122, and 126 of FAC treatment ( p = 35–37). The data is the composite of three independent experiments.

Article Snippet: Western blotting was performed using the following Cell Signaling Technology (Danvers, MA, USA) primary antibodies: DNMT1 rabbit monoclonal (1:1000, #5032), EVI1 rabbit monoclonal (1:500, #2593), Pan-Actin rabbit polyclonal (1:1000, #4968), Acetyl Histone H3 (Lys9/Lys14) rabbit polyclonal (1:1000, #9677), ALDH1A2 rabbit polyclonal (1:1000, #83805), and CRYAB rabbit monoclonal (1:1000, #45844).

Techniques: Over Expression, Marker, Real-time Polymerase Chain Reaction, Transfection, Control, Western Blot, Binding Assay, Sequencing